Fig. 4

Tan IIA activates mitochondrial fission in SW837 cells. a-b Immunofluorescence assay for mitochondria using the mitochondrial specific antibody Tom-20. The mitochondrial fragment was counted to quantify the mitochondrial fission. c-h Western blotting for fission-related proteins. Pro-fission factors such as Drp1, Fis1 and Mff were increased in response to Tan IIA treatment. In contrast, anti-fission factors including Mfn2 and Opa1 were also downregulated in Tan IIA-treated cells. *p < 0.05