Fig. 1
Platelet lysis released sufficient amounts of growth factors in PL. a Schematic overview of preparation of PL. PRP was generated from peripheral blood through a series of centrifugations. Based on platelet counts (109 platelets/ml) after the centrifugation step, sufficient PPP was removed to achieve PRP. PL was prepared from PRP by a simple freeze-thawing procedure. b The quantification of growth factors in PL and PPP was determined using ELISA. Bars represented means ± SD. n = 5; *p < 0.05, **p < 0.01, and ***p < 0.005