Fig. 6

Human DMs with or without centromeres, but lacking telomeres, remained after human chromosome pulverisation. a 5 weeks after fusion of COLO 320DM-donor and MEF acceptor cells, metaphase chromosome spreads were prepared and simultaneously hybridised with a human pan-centromeric alpha satellite probe and an Alu probe. Representative image is shown. White and yellow arrows indicate DMs that are centromere-negative or -positive, respectively. b Simultaneous detection of Alu and telomere sequences in hybrid cells 5 weeks after fusion. The telomeric repeat sequence is conserved between human and mouse, and the FISH signal appeared at all chromosome ends. c Numbers of telomere-positive and -negative DMs per cell. Shown is a result from single experiment