BMC Molecular and Cell Biology

Table 3 Relative expression stabilities of genes in the shell gland of laying hens challenged with IBV

From: Genes involved in mitochondrial biogenesis and function may not show synchronised responses to mitochondria in shell gland of laying chickens under infectious bronchitis virus challenge

Gene	Group				P value
	Virus challenge		Time-point (hr)		Virus challenge	Time-point	Interaction
	Yes	No	5	15	Virus challenge	Time-point	Interaction
CS	1.125 ± 0.097	1.050 ± 0.096	0.892 ± 0.092^y	1.283 ± 0.080^x	0.5522	0.0032	0.7135
SDHA	0.966 ± 0.042^b	1.079 ± 0.054^a	1.154 ± 0.026^x	0.891 ± 0.050^y	0.0343	< 0.0001	0.0188
Drp1	1.066 ± 0.063	0.993 ± 0.053	1.191 ± 0.044^x	0.868 ± 0.047^y	0.2619	< 0.0001	0.7039
PGC-1α	1.025 ± 0.053	1.019 ± 0.049	1.089 ± 0.062	0.956 ± 0.030	0.9273	0.0671	0.9236
CYC, S	0.959 ± 0.025	1.041 ± 0.021	0.829 ± 0.037^y	1.247 ± 0.049^x	0.1900	< 0.0001	0.5145
Na ⁺ -K ⁺ ATPase	1.123 ± 0.134	1.237 ± 0.165	0.692 ± 0.077^y	1.668 ± 0.120^x	0.3916	< 0.0001	0.0087

Values are normalised relative quantities (NRQ) to the mean expression level of all the samples ± S.E. Superscripts (^a,b) across the row indicate significant difference between the virus challenged and control groups. Superscripts (^x,y) across the row indicate significant difference between the 5 and 15 h time-points of egg-shell formation. In each group at each time-point, there were 10 samples processed for qPCR assay

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