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Fig. 2 | BMC Molecular and Cell Biology

Fig. 2

From: Dlx5 and Dlx6 can antagonize cell division at the G1/S checkpoint

Fig. 2

Dlx-expressing C2C12 cells do not show elevated levels of apoptosis. a At the time of seeding the resazurin viability assay, extra C2C12 cells were collected to assess caspase-3 activation levels. Bars represent the average caspase-3 activity, reported as Relative Fluorescence Units per minute per microgram (RFU/min./μg), +/− the standard error of the mean (SEM). b C2C12 cells were transfected and selected in the same manner as for the proliferation assay (n = 2). After 4 days of selection, cells were seeded into 6-well plates in triplicate, at a density of 1.9 × 105 cells/well. At 24, 48 and 72 h-post seeding, floating and adherent cells were collected and extracts assayed for caspase-3 activity. Bars represent the average caspase-3 activity (RFU/min./μg) +/− SEM. There was no significant difference in caspase-3 activation in any Dlx5- or Dlx6-transfected populations compared to the vector control at any time point (ANOVA). c, d Proliferating C2C12 cells were transiently transfected during seeding onto poly-D-Lysine-coated coverslips and double strand DNA breaks were detected 24 h post-transfection. (c) Representative pictures taken with a 40x objective lens. Insets are examples of Dlx-positive and TUNEL-positive cells. d The proportion of Dlx-expressing cells undergoing apoptosis was compared to the surrounding Dlx-negative population. Bars represent two experiments ± SEM, with at least 100 Dlx-positive cells counted in each. No significant differences in % TUNEL labeling were detected (ANOVA)

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