Fig. 1

Lamin A/C and Emerin knockdowns in DLD-1 cells. a Representative western blot showing single or combined knockdowns of Lamin A/C, Emerin in DLD-1 cells. Control: non-targeting siRNA (siLacZ), Loading control: GAPDH. b Densitometric quantification of band intensities for Lamins A, C and Emerin (N = 5 independent biological replicates, *p < 0.05). This shows ~ 75–80% knockdown of Lamin A/C and Emerin in both single and co-depletions respectively c Immunostaining of Lamin A (green) and Emerin (red) in single and co-depleted DLD-1 cells. Scale bar ~ 10 μm, (N = 2, data compiled from two independent biological replicates (N))