Fig. 3

Increase in actin stress fiber aggregates upon Lamin A/C-Emerin co-depletion. a Phalloidin staining (green) in control (untreated and siLacZ) cells, and upon single and co-depletion of Lamin A/C and Emerin. Scale bar ~ 10 μm. Enlarged: Zoomed in images. b % of cells with actin aggregates (from a), n = number of cells analysed, Error bars: SEM, N = 3, ***p < 0.0001 (N: number of independent biological replicates). c Representative western blot showing Actin levels in nuclear extracts of DLD-1 cells with single and combined depletion of Lamin A/C-Emerin d Representative western blot showing Actin levels in cytoplasmic extract of DLD-1 cells upon single and combined depletion of Lamin A/C-Emerin e Densitometric quantification of band intensity for Actin levels in nuclear and cytoplasmic extracts from cells with single and combined depletion of Lamin A/C-Emerin. Error bar: SEM, data compiled from N = 3 independent biological replicates, *p < 0.05