Fig. 2

Impact of miRNA overexpression on endogenous mRNA and protein levels of NDRG2 under basal conditions. Time-course in hours (h) of Ndrg2 mRNA expression following transfection of (a) miR-23a (dark grey bar), (b) miR-23b (light grey bar), and (c) miR-28 (white bar) compared to negative control (NC) miRNA (black bar). NDRG2 protein levels at (d) 12 h, (e) 24 h and (f) 48 h post miRNA transfection. Data are representative of two to three independent experiments with n = 3-4 per sample group, and expression levels are presented as arbitrary units (AU)