Fig. 5

Effects of recombinant SAA1 protein on inflammatory factors in LPS-induced VSMCs detected by qRT-PCR (n = 3). Notes: a, The mRNA expressions of inflammatory factors (IL-1β, IL-6, IL-8, IL-17, TNF-α, MCP-1 and bFGF) in VSMCs after pre-incubation with different concentrations of SAA (5, 10, 15, 20 μg/ml) for 24 h followed by the addition with 1 μg/ml LPS for 24 h; Some VSMCs were treated with SAA (20 μg/ml) alone for 24 h; b, The mRNA expressions of inflammatory factors in VSMCs (IL-1β, IL-6, IL-8, IL-17, TNF-α, MCP-1 and bFGF) after pre-incubation with 20 μg/ml SAA for different time points (0, 24, 48, 72 h) and addition with 1 μg/ml LPS for 24 h. The same letters represented no significant differences, P > 0.05; and different letters indicated significant differences, P < 0.05