Fig. 2

The effect of high glucose stimulation on cell survival and apoptosis in MPC-5 cells. The experiments were divided into three groups, which were control (5.5 mmol/L normal glucose), NM (5.5 mmol/L glucose+ 24.5 mmol/L mannitol) and HG (30 mmol/L glucose). a Cell viability was detected for 24 h and 48 h by CCK-8 assay. b CFSE assay was used to detect cell survival. c Relative apoptosis rate showed as bar diagrams. d Apoptosis levels were detected for 24 h and 48 h by flow cytometry. Data were expressed as mean ± SD from three independent experiments. (*Compared with control, *P < 0.05, **P < 0.01)