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Fig. 1 | BMC Molecular and Cell Biology

Fig. 1

From: Comparison of polymerization and structural behavior of microtubules in rat brain and sperm affected by the extremely low-frequency electromagnetic field

Fig. 1

Microtubule polymerization and tubulin purification. a The effect of 50 Hz ELEF on microtubule polymerization. b Polymerization of microtubule in brain control, brain ELEF, sperm control, and sperm ELEF. c Obtained fractions subjected to SDS-PAGE. The lanes are as follows: tubulin dimers prepared by one cycle of polymerization and depolymerization from sperm extracts (lane 1), α/β tubulin dimers purified by phosphocellulose column chromatography from sperm extracts (lane 2), tubulin dimers prepared by one cycle of polymerization and depolymerization from brain extracts (lane 4), α/β tubulin dimers purified by phosphocellulose column chromatography from brain extracts (lane 3), and protein marker (lane 5). d Polymerization/depolymerization cycle of microtubules shows the native function of microtubule dynamics. The assigned letters of a, b, c, and d denote significant differences (P < 0.05) among the groups. The result is from 3 independent experiments. Values are expressed as mean ± SD, Tukey post hoc test

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