Fig. 2

Western blotting analyses of sucrose density fractions. a-e Representative results of western blotting analyses of sucrose density fractions of undifferentiated (UD) and neural differentiated (Neu) P19C6 cells, treated with or without MβCD. The fractions were incubated with antibodies against Flot2 (a), Fyn (b), c-Src (c), Fyn (pY528) and/or c-Src (pY530) (d), and NKA (e). Cropped images are shown. f-i Densitometric intensity analyses of the bands shown in a–d, representing the relative levels of Flot2 (f), Fyn (g), c-Src (h), and Fyn (pY528) and/or c-Src (pY530) (I) in the DRM (Frs. 4–6) and non-DRM fractions (Frs. 7–12) of UD and Neu cells. Data are represented as the mean ± SEM (n = 6); *p < 0.05 by a two-tailed unpaired Student’s t-test. Abbreviations: Flot2: Flotillin2; NKA: Na/K ATPase; DRM: Detergent-resistant membrane; MβCD: methyl-β-cyclodextrin