Fig. 1

YAP is highly expressed in EOC and is directly regulated by V-ATPase D1 expression. Immunohistochemical observation of V-ATPase D1 and YAP protein expression in normal ovarian epithelium and EOC tissues, a V-ATPase D1 and b YAP (with extremely weak staining (0), a weak staining (+ 1), a moderate staining (+ 2), a strong staining (+ 3). All photographs were taken at original 200 × magnification. The scores of (a) V-ATPase D1 and (b) YAP by beeswarm plot was shown in the bottom (**P < 0.01). c The mRNA level of V-ATPase D1 and YAP in normal ovarian epithelium and EOC tissues were determined using a real-time PCR kit. Data represent the mean and standard deviation (mean ± SD) are from three independent experiments (*P < 0.05). d V-ATPase D1 and YAP expression assessed by Q-PCR analysis in EOC cell lines. Data (mean ± SD) are from three independent experiments (*P < 0.05). e V-ATPase D1, YAP and p-YAP protein levels in A2780/T cells were performed by western blot. f Knocking down the expression of V-ATPase D1 in A2780/T cells impaired the transcriptional activity of YAP in the luciferase assay. g Knocking down the expression of V-ATPase D1 in A2780/T cells impaired the binding of YAP to the promoter region of CTGF and Cyr61