Fig. 3

Effects of acidic pHe on V-ATPase D1 change. a Total protein from PTX- resistant EOC cells and respective sensitive phenotypes were immuno-blotted with anti-V-ATPase D1. b Confocal microscopy showing V-ATPase D1 (green dots) and plasma membrane (red/orange staining) in A2780 and A2780/T cells. DAPI in blue color represents nuclear staining. Merged images (yellow regions). Original magnification× 600. Representative images from three independent experiments are shown. c Cytosolic pH was estimated using pH sensitive dye BCECF-AM. Significantly decreased intracellular pH was observed in EMSO co-treated with PTX in A2780/T cells using BCECF-AM and fluorescence microscopy (488/535 nm, Magnification× 600). d The corresponding intracellular pH was obtained from the pH calibration curve. Values are means ± S.E.M of two independent experiments performed in triplicate (*P < 0.05)