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Fig. 3 | BMC Molecular and Cell Biology

Fig. 3

From: Construction of strains to identify novel factors for regulation of centromeric cohesion protection (CCP) and sister kinetochore mono-orientation (SKM)

Fig. 3

Spo13 and Cdc5 overexpression affect the viability of cells. a Frogging assay of CRY1 integrated with pRS405-URA (SGY4104), pRS405-REC8 (SGY4108), pRS405-SPO13 (SGY4103) and pRS405-REC8-SPO13 (SGY4110) respectively (control, Rec8, Spo13, Rec8Spo13) were first inoculated at 30 °C for overnight in 5 ml SC-uracil broth supplemented with raffinose, now the OD600 of the broth was checked, make the OD600 of each broth ~ 1 by dilution using same broth, then make the dilution and spot them on the SC-URA supplemented with galactose (right panel) or dextrose (left panel) as a carbon source. b Frogging assay of CRY1 integrated with pRS405-URA (SGY4104), pRS405-MAM1 (SGY4106), pRS405-CDC5 (SGY4105) and pRS405-MAM1-CDC5 (SGY4107) respectively (control, Mam1, Cdc5, Mam1Cdc5) on SC-uracil plate supplemented with galactose (right panel) or dextrose (left panel) similarly as mentioned earlier. c Cell morphology of SGY4112 (GAL-SPO13) strain in dextrose and galactose

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