Fig. 8
From: Design and structural characterisation of olfactomedin-1 variants as tools for functional studies
SAXS analysis indicates Olfm1BMY is an “X”-shaped tetramer. a Averaged and reference-subtracted SAXS data of Olfm1BMY at 0.615 mg/mL plotted as Log I vs Q. b Guinier plot of the SAXS data indicates Olfm1BMY forms a tetramer (Rg of 5.4 nm and I0 corresponding to 84.0 kDa). c The Kratky plot suggests Olfm1BMY has substantial flexibility yet is more structured than a random coil. d The pair-distance distribution function P(r) has an asymmetric bell shape with a maximum at 4.76 nm, and a maximum diameter (Dmax) of 16.3 nm. e The fit to the data (red circles) of the P(r) (black line) and the theoretical scattering of the ab-initio bead model by Dammif shown in panel f (green dashes) indicate a good agreement with the data (χ2 of 3.34 for the P(r) and 1.24 for the ab-initio model). f Dammif ab-initio model of Olfm1BMY with enforced C2 symmetry reveals a shape similar to what was predicted based on the architecture of the longer BMZ isoform [6] (see panel g). g Previously-published slice through a negatively stained electron tomogram of Olfm1BMZ [6], with the region corresponding to Olfm1BMY indicated by a red ellipse