Fig. 7

Graphical conclusions. Antibody folding is a complex process which occurs in vivo and where light chains are coupled to previously assembled heavy chain dimers. In this work, denaturation and chains separation of the Trastuzumab antibody followed by their in vitro refolding through slow dialysis method has shown that heavy chain dimers stabilized by disulphide bridges are necessary in order to reassemble the whole antibody. Successful in vitro assembly of heavy and light chains has been achieved when the chains have been independently produced