Fig. 4

Distribution and colocalization of proCtsK and TRAP isoforms in MDA-MB-231cells. a Coefficient of variation of Alexa 488 signal was calculated to analyze distribution of proCtsK signal in the cell. Means (8–20 cells in at least 3 fields of view) from 3 independent experiments were statistically compared by t-test (n = 3 cultures). b Coefficient of variation of Alexa 647 signal (B) was calculated to analyze distribution of TRAP 5a and total TRAP signals over the cell. Means from independent experiments were statistically compared by one-way ANOVA. c Colocalization of proCtsK and uncleaved TRAP 5a vs. proCtsK and total TRAP was analyzed by calculation of a Pearson’s correlation coefficient (PCC). Means from independent experiments were statistically compared by one-way ANOVA with Tukey’s correction for multiple comparisons. * p < 0.05, ** p < 0.01 *** p < 0.001