Fig. 6

TRAP isoform fractioning in MDA-MB-231 cells after CtsK inhibition.a Fast protein liquid chromatography (FPLC) of cell lysate derived from mock control and/or TRAP3^high cells treated with DMSO or with 100 nM CtsK inhibitor (MK-0822, Odanacatib) on heparin columns and a NaCl gradient from 0.1 M– 1 M NaCl (independent experiments n = 2). Fractions were measured for TRAP activity (represented by absorbance signal at 405 nm). b Respective peaks for FPLC pattern of recombinant TRAP 5a and TRAP 5b protein are denoted. Western blot for total TRAP protein expression. FPLC Fractions of TRAP3^high cells treated with DMSO or with 100 nM CtsK inhibitor (MK-0822, Odanacatib) were measured for TRAP protein expression and denoted as FXX-XX