Fig. 1

Critical parameters for the transfection of BFF. a Effect of electroporation medium (other electroporation conditions: 4 mm cuvette, 1 pulse, 400 V, 10 milliseconds, 5 μg DNA, room temperature handling). HC, handling control; MC, mock control; EPB, Biorad (Gene Pulser) electroporation buffer; Opti-MEM, ThermoFisher medium with GlutaMax; D10, fibroblast cell culture medium; PBS, phosphate buffered saline. b Influence of DNA concentration on transfection efficiency (other electroporation conditions: 4 mm cuvette, 1 pulse, 300 V, 10 milliseconds, opti-MEM as electroporation buffer, room temperature handling). c Influence of pulse duration on transfection efficiency (other electroporation conditions: 4 mm cuvette, 1 pulse, 400 V, 10 μg DNA, opti-MEM as electroporation buffer, room temperature handling). d Influence of pulse voltage on transfection efficiency (other electroporation conditions: 4 mm cuvette, 1 pulse, 5 μg DNA, 10 milliseconds, opti-MEM as electroporation buffer, room temperature handling). All the values are indicated by mean ± SEM. Means bearing different superscripts in upper case differ significantly in terms of viability, and those bearing different superscripts in lower case differ significantly in terms of fluorescence. Number of replicates = 3