Fig. 2
From: Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts
Transfection efficiency in bovine fetal fibroblasts with varying pulse durations. a 1 ms, b 5 ms, c 10 ms, d 20 ms. Brightfield, Hoechst 33342-stained nuclei, Venus fluorescent cells and merged images are shown. Number of replicates = 3. (other electroporation conditions: 4 mm cuvette, 1 pulse, 400 V, 10 μg DNA, opti-MEM as electroporation buffer, room temperature handling)