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Table 1 Effect of pulse number on transfection efficiency

From: Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts

No of pulses

Viability (%)

Fluorescence (%)

1

65.0 ± 0.89a

7.2 ± 0.20 a

2

60.6 ± 0.92b

6.2 ± 0.18 b

3

64.8 ± 0.73a

7.8 ± 0.13 a

  1. Other pulse conditions: 200 V; pulse interval, 1 ms; pulse duration, 10 ms; 2 mm cuvette; 4 μg DNA; number of replicates =3. The electroporation buffer was BioRad electroporation buffer
  2. Different superscript letters indicate significant difference (P < 0.05)
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BMC Molecular and Cell Biology

ISSN: 2661-8850

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