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Table 1 Effect of pulse number on transfection efficiency

From: Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts

No of pulsesViability (%)Fluorescence (%)
165.0 ± 0.89a7.2 ± 0.20 a
260.6 ± 0.92b6.2 ± 0.18 b
364.8 ± 0.73a7.8 ± 0.13 a
  1. Other pulse conditions: 200 V; pulse interval, 1 ms; pulse duration, 10 ms; 2 mm cuvette; 4 μg DNA; number of replicates =3. The electroporation buffer was BioRad electroporation buffer
  2. Different superscript letters indicate significant difference (P < 0.05)