Fig. 5
From: BACE2 degradation is mediated by both the proteasome and lysosome pathways
Both lysosomal inhibition and proteasomal inhibition increases the expression of endogenous BACE2 in HEK293 cells. a HEK293 cells were treated with vehicle (Control) and NH4Cl for 24 h, and MG-132 for 12 h, respectively. Anti-BACE2 antibody was used to detect endogenous BACE2 protein. β-actin served as an internal control and was detected by AC-15 antibody. b Quantification of BACE2 protein levels. Values represent mean ± SEM; n ≥ 3, *P < 0.05 by one-way ANOVA followed by Tukey’s test