Fig. 6
From: Robust high-throughput assays to assess discrete steps in ubiquitination and related cascades
Effects of compounds in the PCNA ubiquitination Alpha assay. Samples were preincubated with compounds for 15 min prior to initiation of reactions with ATP. Data represent mean and SD for 3–7 samples in each case. Final DMSO concentration was 1% in all cases. a Dose–response for bioactive inhibitors of PCNA ubiquitination, plotted both linearly and semi-logarithmically (inset). b Effects of compounds with little activity against PCNA ubiquitination at 500 μM, normalized to positive (DMSO alone) control values