Fig. 5

MALAT1 acts as a sponge for miR-503 (a) Schematic representation of binding sites between MALAT1 and miR-503 predicted by StarBase software. b Wild-type and mutant-type MALAT1 luciferase reporter gene vectors were constructed and named wt-MALAT1 and mut-MALAT1; mut-MALAT1 contained a 4 bp mutation on any of the predicted miR-503 binding sites. The above vectors were co-transfected into HEK-293 cells with miR-NC or miR-503 mimics; the luciferase activity was determined. c NPCs were transfected with MALAT1 siRNA; the expression of miR-503 was determined using real-time PCR. d NPCs were transfected with miR-NC or miR-503 mimics; the expression of MALAT1 was determined using real-time PCR. e The expression levels of miR-503 in IDD tissues and normal ones were determined by RT-qPCR. f The correlation between MALAT1 and miR-503 was analyzed using Spearman’s rank correlation analysis. The data are presented as mean ± SD of three independent experiments. *P < 0.05