Fig. 6
PGRMC1 phosphorylation is associated with NNMT regulation. a Quantification of NNMT mRNA levels in the four cell types by RT-PCR. Quantification cycle (Cq) results were quantified using the 2ΔΔCq method using beta actin as an internal control. ANOVA post-hoc Tukey’s HSD p < 0.01 (**) or p < 0.001 (***). b Model of the NNMT and 1-methylnicotinamide pathway. Nicotinamide is produced from NAD by NAD-dependent deacetylases (e.g. Sirtuins), PARPS or CD38 [51]. NNMT transfers a methyl group of S-adenosylmethionine (SAM) to nicotinamide to produce 1-methylnicotinamide (1-MNA), producing S-adenosyl homocysteine (SAH). This reaction is in competition with methylation reactions including DNA and histone methylation by SAM [52]. 1-MNA can feed back to stabilize sirtuin 1 [53]