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Fig. 2 | BMC Molecular and Cell Biology

Fig. 2

From: Basic biochemical characterization of cytosolic enzymes in thymidine nucleotide synthesis in adult rat tissues: implications for tissue specific mitochondrial DNA depletion and deoxynucleoside-based therapy for TK2-deficiency

Fig. 2

Distribution of cytosolic deoxynucleoside kinases in adult rat tissues determined by anionic (a, c, e, g, i, k and m) and cationic (b, d, f, h, j, l, and n) exchange column chromatography. Cytosolic proteins from each tissue were applied to a anionic exchange (DEAE-sephadex) column and the flow through (unbound protein) was applied to a cationic exchange (CM-sepharose) column. The bound proteins were then eluted with KCl gradient. Fractions were collected and assayed using tritium labeled dT (filled circles) and dC (open circles) as substrate. TK2 and dCK bind to DEAE-Sephadex column and TK2 eluted at low KCl concentration and dCK eluted at higher KCl concentration. TK1 binds to CM-Sepharose column and eluted at low KCl concentration

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BMC Molecular and Cell Biology

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