Fig. 6

Effects of RORα deficiency on NCEH1 and lipase expression and lipid droplet accumulation. a HepG2 cells were transfected with 50 nM siRNA and analyzed by qRT-PCR to measure the expression of RORα and NCEH1. siRNAs targeting sequences in the RORα coding region (siRORα) were used and the resulting RORα and NCEH1 mRNA levels were measured. siRNA targeting green fluorescent protein was used as a negative control (siGFP). Data are means ± standard errors (n = 3) and were normalized to 18S rRNA levels. *, p < 0.05. b Effects of siRNA transfection on the expression of adipose triglyceride lipase (ATGL) and Lipase E (LIPE). c Effects of siRNA transfection on cell viability were estimated by measuring lactate dehydrogenase (LDH) activity in the culture medium of siRNA-transfected cells. Data are means ± standard errors (n = 4). *, p < 0.05. d Lipid droplet accumulation was demonstrated by Oil Red O staining. At 48 h after transfection with siRNA targeting RORα or GFP, HepG2 cells were treated with 400 μM oleic acid or DMSO (as a control) for 24 h. e HepG2 cells were transfected with siRNA and treated with 0, 100, or 400 μM oleic acid for 24 h. Subsequently, the quantification of absorbance was performed to determine Oil Red O staining levels in the cells. Data are means ± standard errors (n = 3). *, p < 0.05