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Fig. 2 | BMC Molecular and Cell Biology

Fig. 2

From: Functional analyses of miRNA-146b-5p during myogenic proliferation and differentiation in chicken myoblasts

Fig. 2

Gene expression analyses of undifferentiated primary chicken myoblast (pCM) cells by quantitative RT-PCR (qRT-PCR) and Western blotting. a mRNA expression profiles of target transcripts of miR-146b-5p (Smad4, Numb, Asck3, Rrm2b, and Sgcb) were compared between regular pCM (rpCM) and pCM cells overexpressing miRNA-146b-5p (pCM-146b OE cells) by qRT-PCR (n = 3; **p < 0.01, ***p < 0.001). b mRNA expression profiles of Pax7 and MyoD were compared between rpCM and pCM-146b OE cells by qRT-PCR (n = 3; **p < 0.01, ***p < 0.001). c Protein expression profiles of PAX7 and MYOD were compared between undifferentiated regular pCM and pCM-146b OE cells. The graph represents a density comparison of Western blotting results (n = 3; *p < 0.05)

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