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Fig. 3 | BMC Molecular and Cell Biology

Fig. 3

From: Functional analyses of miRNA-146b-5p during myogenic proliferation and differentiation in chicken myoblasts

Fig. 3

Proliferation and gene expression analyses during primary chicken myoblast (pCM) cell growth. a Cell proliferation was compared between GFP-expressing pCM (pCM-GFP cells) and pCM cells overexpressing miRNA-146b-5p (pCM-146b OE cells) over 5 days (n = 3; **p < 0.01, ***p < 0.001). The right panels show flow cytometry analyses with BrdU for cell cycles, indicating that subsets of pCM-146b OE cells in S phase were significantly increased compared to rpCM cells (rpCM cells: 22.6 ± 0.9% vs. pCM-146b OE cells: 47.8 ± 6.0%, n = 3). b mRNA expression profiles of Ccnd3, Irf2, Wnt5a, and Pdgfrb were compared between regular pCM and pCM-146b OE cells by qRT-PCR (n = 3; **p < 0.01, ***p < 0.001)

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BMC Molecular and Cell Biology

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