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Table 2 Primers used for the RT-PCR

From: The local hypothalamic–pituitary–adrenal axis in cultured human dermal papilla cells

Gene PCR primer sequence Product size (bp)
Actin F 5′- GCG TGA CAT TAA GGA GAA GC -3′ 174
R 5′- AGG AAG GAA GGC TGG AAG A -3′
Wnt5a F 5′- CCA CAC AAG ACC TGG TCT ACA TC -3′ 172
R 5′- GTC TGC ACG GTC TTG AAC TG -3′
VEGF F 5′- TAC CTC CAC CAT GCC AAG T -3′ 343
R 5′- TGC ATT CAC ATT TGT TGT GC -3′
HGF F 5′- TGC AGA GGG ACA AAG GAA AAG -3′ 378
R 5′- GAA CTC CAG GGC TGA CAT TTG -3′
TGFβ2 F 5′- AGG GTA CAA TGC CAA CTT CTG -3′ 144
R 5′- GGT TCT AAA TCT TGG GAC ACG -3′
Versican F 5′- TGA CTG TGG ATG GGG TTG TG -3′ 141
R 5′- GCG TCA CAC TGC TCA AAT CC -3′
ALP F 5′- CCA AGG ACG CTG GGA AAT CT -3′ 172
R 5′- TAT GCA TGA GCT GGT AGG CG -3′
POMC F 5′- CCC CTA CAG GAT GGA GCA CT -3′ 116
R 5′- GGC GTT TTT GAA CAG CGT CA -3′