Table 2 Primers used for the RT-PCR
From: The local hypothalamic–pituitary–adrenal axis in cultured human dermal papilla cells
Gene | PCR primer sequence | Product size (bp) |
|---|---|---|
Actin | F 5′- GCG TGA CAT TAA GGA GAA GC -3′ | 174 |
R 5′- AGG AAG GAA GGC TGG AAG A -3′ | ||
Wnt5a | F 5′- CCA CAC AAG ACC TGG TCT ACA TC -3′ | 172 |
R 5′- GTC TGC ACG GTC TTG AAC TG -3′ | ||
VEGF | F 5′- TAC CTC CAC CAT GCC AAG T -3′ | 343 |
R 5′- TGC ATT CAC ATT TGT TGT GC -3′ | ||
HGF | F 5′- TGC AGA GGG ACA AAG GAA AAG -3′ | 378 |
R 5′- GAA CTC CAG GGC TGA CAT TTG -3′ | ||
TGFβ2 | F 5′- AGG GTA CAA TGC CAA CTT CTG -3′ | 144 |
R 5′- GGT TCT AAA TCT TGG GAC ACG -3′ | ||
Versican | F 5′- TGA CTG TGG ATG GGG TTG TG -3′ | 141 |
R 5′- GCG TCA CAC TGC TCA AAT CC -3′ | ||
ALP | F 5′- CCA AGG ACG CTG GGA AAT CT -3′ | 172 |
R 5′- TAT GCA TGA GCT GGT AGG CG -3′ | ||
POMC | F 5′- CCC CTA CAG GAT GGA GCA CT -3′ | 116 |
R 5′- GGC GTT TTT GAA CAG CGT CA -3′ |