Fig. 2
From: Obg-like ATPase 1 inhibited oral carcinoma cell metastasis through TGFβ/SMAD2 axis in vitro
Dysregulation of OLA1 affected the ability of metastasis in oral cancer cells. a Wound healing assay to examine the effect of down regulating OLA1 on UM-1 and UM-2 cells (n = 3). b Transwell assay to investigate the impact of downregulating OLA1 on metastatic ability in UM-1 and UM-2 cells (n = 3). c Construction of stable cell lines with UM-1 (YFP-OLA1OE) plasmid or lentiviral vector in UM-2 (GFP-shOLA1). Top panel: Fluorescent photos of indicated cells. Bottom panel: OLA1 expression in relatived cells. d Morphology of cells (200X) after OLA1 plasmid transfection or TGFβ1 (1 ng/mL) induction. e Wound healing assay to examine the effect of upregulating OLA1 on UM-1 and downregulating OLA1 on UM-2 cells (n = 3). f Transwell assay to examine the effect of upregulating OLA1 on UM-1 and downregulating OLA1 on UM-2 cells (n = 3). vs. Control, *P < 0.05, **P < 0.01, ***P < 0.001