Fig. 11From: A fast, efficient and high-throughput procedure involving laser microdissection and RT droplet digital PCR for tissue-specific expression profiling of rice rootsddRT-PCR for 5NG4 A), EXP’ B) and OsSHR1 C) using serial dilutions of total root RNA. Left, 1D diagram of ddRT-PCR for 5NG4, EXP’ and OsSHR1 in serial dilutions of total root RNA ranging from 1 ng to 1 fg and in a negative control (H2O). Right, diagrams showing the correlations of the percentages of positive droplets or copies per microliter with the amount (pg) of RNA per reaction. Red bar, threshold for droplet detectionBack to article page