Table 2 Mean 2-Cq, Standard Deviation (S.D)Â 2-Cq and CV% of the candidate reference genes
Candidate Reference Gene | MCF-7 Replicate Culture | Mean 2-Cq | S.D 2-Cq | CV (S.D/Mean) |
|---|---|---|---|---|
ACTB | A1 | 1.6E-05 | 2.9E-06 | 18.81% |
A2 | 1.4E-05 | 3.3E-06 | 22.98% | |
GAPDH | A1 | 7.0E-06 | 8.7E-07 | 12.35% |
A2 | 7.5E-06 | 2.0E-06 | 26.84% | |
RPL13A | A1 | 4.7E-07 | 8.7E-08 | 18.39% |
A2 | 5.9E-07 | 1.6E-07 | 26.71% | |
PGK1 | A1 | 4.5E-07 | 6.7E-08 | 14.86% |
A2 | 4.9E-07 | 2.0E-07 | 40.11% | |
HSPCB | A1 | 7.4E-07 | 1.4E-07 | 19.34% |
A2 | 7.5E-07 | 2.4E-07 | 31.31% | |
RNA28S | A1 | 3.3E-03 | 5.8E-04 | 17.66% |
A2 | 3.1E-03 | 7.7E-04 | 24.52% | |
RNA18S | A1 | 4.2E-03 | 8.0E-04 | 19.17% |
A2 | 3.4E-03 | 9.9E-04 | 28.97% | |
PUM1 | A1 | 1.1E-07 | 1.7E-08 | 15.65% |
A2 | 1.2E-07 | 2.9E-08 | 25.03% | |
CCSER2 | A1 | 1.0E-08 | 1.6E-09 | 15.55% |
A2 | 1.0E-08 | 2.7E-09 | 25.65% | |
HNRNPL | A1 | 1.5E-07 | 3.5E-08 | 23.70% |
A2 | 1.4E-07 | 5.2E-08 | 38.21% | |
PCBP1 | A1 | 2.2E-07 | 3.3E-08 | 14.89% |
A2 | 2.2E-07 | 5.5E-08 | 24.90% | |
SF3A1 | A1 | 9.3E-08 | 2.0E-08 | 21.20% |
A2 | 8.7E-08 | 2.4E-08 | 27.76% |