Fig. 5
From: ESCRT machinery plays a role in microautophagy in yeast
Detection of degradation products of Sna4-GFP. Cells of strains SCU2684 (wild-type; BY4741), SCU5456 (vps27∆), SCU6187 (vps28∆), SCU6188 (vps36∆), and SCU4337 (vps24∆) harboring plasmid pSCU2475 (pSNA4-GFP) were treated with rapamycin for 6 h. Whole cell extracts were subjected to western blotting using the anti-GFP antibody. To assess microautophagic flux, the ratios of cleaved free GFP versus Pgk1 were calculated and the values relative to that in wild-type cells are shown. The average and standard deviation were determined from three independent experiments, and relative values were normalized against the value in control cells are shown. p-values were calculated using two-way ANOVA with Bonferroni correction. *, p < 0.05; **, p < 0.005