Fig. 5
Fluorescence microscopy showing the dependency of filament formation of MreB paralogs on protein concentration, on a planar membrane. a Different concentrations of isolated YFP-MreB monomers after addition of different concentrations MgCl2, or of CaCl2, as stated above and below the panels, (b) YFP-MreB (2 μM) after addition of 10 mM MgCl2, in the presence of different concentrations of KCl as stated above the panels, (c) Different concentrations of CFP-Mbl or of mCherry-MreBH (after strep-purification) as stated above the panels after addition of 10 mM MgCl2, white bars 2 μm