Fig. 1
From: Scl-Ab reverts pro-osteoclastogenic signalling and resorption in estrogen deficient osteocytes
The effect of CM from OCY454 cells on osteoclastogenesis and bone resorption. Images show (a) multinucleated TRAP+ osteoclasts (orange arrows) (N = 3, n = 9) and b bone resorption pits and trails (orange arrows) (N = 2, n = 8) after BMM cells were treated with CM from OCY454 cells. Quantification of images showing (c) percentage surface area covered by TRAP+ multinucleated cells (N = 3, n = 9), d TRAP activity in supernatant collected from multinucleated cells (N = 3, n = 9) and e percentage area resorbed by osteoclasts (N = 2, n = 8) (‘+’ indicates a significant interaction (p < 0.001) between the inhibitory effects of estrogen and Scl-Ab on bone resorption as determined by two-way ANOVA). E, estrogen treatment; ED, Estrogen deficient; Scl-Ab, Sclerostin antibody. Student’s t-test. * = p < 0.05, ** = p < 0.01 and **** = p < 0.0001