Fig. 4
From: Scl-Ab reverts pro-osteoclastogenic signalling and resorption in estrogen deficient osteocytes
The effect of estrogen deficiency and sclerostin inhibition on RANKL and OPG gene expression. qRT-PCR analysis of gene expression (relative to estrogen treated group) in OCY454 cells 24 h after mechanical stimulation (a) RANKL expression (N = 3, n = 9), b OPG expression (N = 3, n = 9) and c RANKL to OPG ratio (N = 3, n = 9). (‘a’ and ‘b’ indicates a significant interaction (p < 0.05 and p < 0.01 respectively) between the effects of estrogen and Scl-Ab on RANKL and OPG expression as determined by two-way ANOVA) E, estrogen treatment; ED, Estrogen deficient; Scl-Ab, Sclerostin antibody. Student’s t-test. * = p < 0.05, ** = p < 0.01 and **** = p < 0.0001