Fig. 2

Arecoline promotes fibroblasts proliferation by inducing Wnt5a expression. a Human oral fibroblasts were treated with or without 8 μg/ml Arecoline for indicated times, then cell lysates were analyzed by Western blotting using indicated antibodies. b-d Human oral fibroblasts were treated with 8 μg/ml Arecoline, recombinant Wnt1, Wnt2 or Wnt5a protein and Wnt1, Wnt2 or Wnt5a antibody for 48 h, then cell proliferation rate was quantified. e Human oral fibroblasts were treated with 8 μg/ml Arecoline, Wnt1, Wnt2 or Wnt5a sepecific siRNAs for 48 h, then cell proliferation rate was quantified. * denotes p < 0.05