Fig. 6

PPP1R2 mutant overexpression increased midbody length. a ARPE-19 cells were transfected with FLAG, PPP1R2, and PPP1R2 mutants (R2A, R2E, R2ΔC, R2ΔN), fixed, and labeled with DAPI (blue) as well as α-tubulin (red) to stain the central spindle. Size bar equals 5 μm. b Midbody length was quantified using Metamorph software and averages were calculated within three biological replicates (n ≥ 30 dividing cells). Statistically significant differences (p ≤ 0.05) between experimental groups and empty vector control are indicated by differing letters above the bars and asterisks indicate *p ≤ 0.01 and **p ≤ 0.001