Table 1 Antibodies used in the study
Antibody (clone) | Target | Company/Reference | Catalog # | Working concentration |
|---|---|---|---|---|
Flow cytometry antibodies and isotype controls | ||||
 CD45-PE (OX-1) | CD45, PTPRC | Novus Biologicals | NB100–64895PE | 0.85 μg/ million cells |
 PE Mouse IgG1 | IgG1 κ isotype control | BD Pharmingen | 555749 | 0.2 μg/ million cells |
 HSECa antibody (SE-1) -AF488 | CD32b, FcγRIIb2 | Novus Biologicals | NB110–68095AF488 | 1 μg/ million cells |
 Mouse IgG2a AF488 (MG2a-53) | IgG2a κ isotype control | Novus Biologicals | NB600-986AF488 | 0.65 μg/ million cells |
 CD31-eFluor 660 (TLD-3A12) | CD31, PECAM-1 | eBioscience | 50–0310-82 | 0.2 μg/ million cells |
 Mouse IgG1k- eFluor 660 (P3.6.2.8.1) | IgG1 κ isotype control | eBioscience | 50–4714 | 0.2 μg/ million cells |
Immune staining of cells and tissues | ||||
 HSECa antibody (SE-1) | CD32b, FcγRIIb2 | Novus Biologicals | NB110–68095 | 10 μg/ml |
 CD11b/c Biotin (OX-42) | CD11b/c, CR3 | Cedarlane | CL042B | 2 μg/ml |
 CD163 (ED2) | CD163 | AbD Serotec | MCA342GA | 10 μg/ml |
 CD68 (ED-1) | CD68 antigen, macrosialin | Abcam | ab31630 | 20 μg/ml |
 GFAP | Glial fibrillary acidic protein | Dako | Z0334 | 15 μg/ml |
 Human MMR/CD206 | CD206, macrophage mannose receptor | R&D Systems | AF2534 | 2 μg/ml |
 SR-A1/MSR | Macrophage scavenger receptor A1 | Novus Biologicals | NBP1–00092 | 12 μg/ml |
 SR-B1 | Scavenger receptor B1 | Novus Biologicals | NB400–104 | 10 μg/ml |
 Rabbit anti-rat HA/SR serumb | Stabilin-2, STAB2 | (24) |  | 1:200 |
 CD45 (OX-1) | CD45, PTPRC | Novus Biologicals | NB100–64895 | 10 μg/ml |
 CD31 (TLD-3A12) | CD31, PECAM-1 | Invitrogen | MA1–81051 | 10 μg/ml |
Magnetic-activated cell sorting | ||||
 HSECa antibody (SE-1) | CD32b, FcγRIIb2 | Novus Biologicals | NB110–68095 | 0.2 μg/million NPCs |
 CD11b/c Biotin (OX-42) | CD11b/c, CR3 | Cedarlane | CL042B | 0.1 μg/million NPCs |
 Anti-Mouse IgG2a + b MicroBeads | IgG2a + b | Miltenyi | 130–047-201 | 2 μl/million NPCs |