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Fig. 7 | BMC Molecular and Cell Biology

Fig. 7

From: Regulation of the Fasciola hepatica newly excysted juvenile cathepsin L3 (FhCL3) by its propeptide: a proposed ‘clamp-like’ mechanism of binding and inhibition

Fig. 7

Inhibition profile of wild-type and variant FhCL3 propeptides against recombinant F. hepatica cathepsin L peptidases. The inhibitory activity of wild-type ppFhCL3 and variant ppFhCL3_Tyr46Lys47/Ala46Ala47, ppFhCL3_Leu66/Gly66 and ppFhCL3_Glu68/Arg68 was examined at 10 nM against recombinant cathepsin L peptidases FhCL1, FhCL2 and FhCL3. The inhibitory activities are presented relative to the total activity of each enzyme alone. The experiments were performed in triplicate and the results are presented as mean ± standard deviation. Statistical analysis was carried out using One-way ANOVA with Dunnett multiple comparison (* P < 0.05, *** P ≤ 0.001)

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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