Fig. 2

Serum starvation effects on expression pattern and ratio of BRCA2 5′ UTR splicing variants. a RT-PCR products between canine BRCA2 exon 1 and exon 2 in four canine cell lines with or without 72-h serum starvation were electrophoresed. RT indicates RT-PCR products and non-RT indicates PCR products of the templates without reverse transcription. SV and RS indicate splicing variant and registered sequence, respectively. b Relative expression ratio of registered sequence (ordinary exon 1–2) versus total canine BRCA2 expression (exon 26–27) in four canine cell lines was measured by quantitative PCR. To quantify registered sequence, the exon 1 and 2 spanning primer was used. c Relative expression level of canine BRCA2 normalized by canine RPS18 in four canine cell lines. “**” indicates p < 0.01