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Fig. 6 | BMC Molecular and Cell Biology

Fig. 6

From: Reduced translation efficiency due to novel splicing variants in 5′ untranslated region and identification of novel cis-regulatory elements in canine and human BRCA2

Fig. 6

Effect of human BRCA2 intron 1 region on transcription activity. Human BRCA2 intron 1 region was inserted upstream of human BRCA2 promoter region (− 187 − + 310 bp) to measure the transcriptional activity using dual-luciferase reporter assay. a The promoter activities of the entire intron 1 region without promoter (+ 311 − + 942 bp) and conserved central 500 bp (+ 443 − + 842 bp) were measured. The promoter activities of sequential 100 bp deletion from 3′ end (b) and 5′ end (c) were compared. d and e To analyze in more detail the cis-regulatory elements in human BRCA2 intron 1, the indicated 100 bp of human BRCA2 intron 1 was inserted upstream of human BRCA2 promoter and compared. “*” indicates p < 0.05 compared with the construct that only had the BRCA2 promoter. “N. S.” indicates not significant.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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