Fig. 1

circ_0005529 was up-regulated in gastric carcinoma tissues and cells. a Volcano plot of differentially expressed circRNAs. Upregulated and downregulated circular RNAs were screened (red: upregulated, blue: downregulated). b Heatmap showing the top 22 significant disordered circular RNAs in GEO: GSE78092; circ_0005529 was upregulated in GC tissues. T-test analysis generated miRNAs that were overexpressed (red, change scale: 0 to 1) and decreased (green, change scale: − 1 to 0) in the GC samples compared to the adjacent samples (0 represents no change). c Relative circ_0005529 expression levels in GC tissues (tumor, n = 63) and normal adjacent tissues (adjacent, n = 63), as determined using qRT-PCR. d Kaplan-Meier curves of overall survival of 63 GC patients, stratified by circ_0005529 expression. e Relative circ_0005529 expression levels in human normal gastric mucosal epithelial cells (GES-1) and four gastric cancer cell lines (HGC-27, MGC803, AGS and MKN-45), as determined by qRT-PCR. f Relative linear VPS33B mRNA and circ_0005529 expression levels in HGC-27 and MKN-45 cells after RNase R treatment, as determined using qRT-PCR. g RNA fluorescence in situ hybridisation for circ_0005529 and Actin was performed in HGC-27 and MKN-45 cells (green, circ_0005529; red, Actin; blue, U6). (Mean ± SEM, ** p < 0.01)