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Table 7 List of primary antibodies

From: Global transcriptome profile of the developmental principles of in vitro iPSC-to-motor neuron differentiation

Antibody

Species

Dilution

Source

Oct4

Rabbit

1:200

Cell Signaling Technology (Cat# 2750S)

Nestin

Rabbit

1:200

Abcam (Cat# ab105389)

Pax6

Mouse

1:50

Developmental Studies Hybridoma Bank (Cat# Pax6)

MAP2

Mouse

1:200

ThermoFisher (Cat# MA5–12823)

HB9 / MNX1

Mouse

1:50

Developmental Studies Hybridoma Bank (Cat# 81.5C10)

ChAT

Goat

1:100

Milipore (Cat#AB144P)

beta-III-tubulin

Rabbit

1:1000

Abcam (Cat# ab18207)

Synaptophysin [SY38]

Mouse

1:100

Abcam (Cat# ab8049)