Fig. 9From: Isolation, culture, and characterization of chicken intestinal epithelial cellsZO1 and VIM expression by double staining IF. In this reaction the same sample was stained with two primary antibodies (ZO1 and VIM) at the same time, to prove the absence of VIM expression in ZO1 positive cIECs (11d after seeding). a) ZO1 (FITC+DAPI - 100% positive); b VIM (TRITC+DAPI - negative)Back to article page