Table 1 Primers used in RT-qPCR
| Gene (human) | Forward and reverse primers (5′-NNN-3′) |
|---|---|
| GAPDH |
F: 5′-TCGACAGTCAGCCGCATCTTCTTT-3′ R: 5′-ACCAAATCCGTTGACTCCGACCTT-3’ |
| Glypican-1 |
F: 5’-TATTGCCGAAATGTGCTCAAGGGC-3′ R: 5′-ATGACACTCTCCACACCCGATGTA-3’ |
| Glypican-2 |
F: 5’-TCCTTTCTGGTTCACACACTGGCT-3′ R: 5′-ACAGGCCATTGAATATGAGGGCGT-3’ |
| Glypican-3 |
F: 5’-TGAAAGTGGAGACTGCGGTGATGA-3′ R: 5′-TCCCGAGGTTGTGAAAGGTGCTTA-3’ |
| Glypican-4 |
F: 5’-TCGGAGATGTCCCTCGCAAATTGA-3′ R: 5′-TCTTCAACAGGGCATGGGTACACT-3’ |
| Glypican-5 |
F: 5’-ACTGGCATGCATATATCCGGTCGT-3′ R: 5′-TGAGGTGAGCCTGTAACACAGCAT-3’ |
| Glypican-6 |
F: 5’-CTTCATTGCTGCCAGGACCTTTGT-3′ R: 5′-AGCCCTTCATGACGTTGAGACAGT-3’ |
| β-catenin |
F: 5’-CTTCACCTGACAGATCCAAGTC-3′ R: 5′-CCTTCCATCCCTTCCTGTTTAG-3’ |