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Table 1 Primers used in RT-qPCR

From: The Glypican proteoglycans show intrinsic interactions with Wnt-3a in human prostate cancer cells that are not always associated with cascade activation

Gene (human) Forward and reverse primers (5′-NNN-3′)
GAPDH F: 5′-TCGACAGTCAGCCGCATCTTCTTT-3′
R: 5′-ACCAAATCCGTTGACTCCGACCTT-3’
Glypican-1 F: 5’-TATTGCCGAAATGTGCTCAAGGGC-3′
R: 5′-ATGACACTCTCCACACCCGATGTA-3’
Glypican-2 F: 5’-TCCTTTCTGGTTCACACACTGGCT-3′
R: 5′-ACAGGCCATTGAATATGAGGGCGT-3’
Glypican-3 F: 5’-TGAAAGTGGAGACTGCGGTGATGA-3′
R: 5′-TCCCGAGGTTGTGAAAGGTGCTTA-3’
Glypican-4 F: 5’-TCGGAGATGTCCCTCGCAAATTGA-3′
R: 5′-TCTTCAACAGGGCATGGGTACACT-3’
Glypican-5 F: 5’-ACTGGCATGCATATATCCGGTCGT-3′
R: 5′-TGAGGTGAGCCTGTAACACAGCAT-3’
Glypican-6 F: 5’-CTTCATTGCTGCCAGGACCTTTGT-3′
R: 5′-AGCCCTTCATGACGTTGAGACAGT-3’
β-catenin F: 5’-CTTCACCTGACAGATCCAAGTC-3′
R: 5′-CCTTCCATCCCTTCCTGTTTAG-3’