Fig. 8
From: Chick fetal organ spheroids as a model to study development and disease
Confrontation assay. a-l Fetal brain and fetal lung spheroids (FBSs and FLuSs) were cultured separately in confrontation assays together with spheres of GFP-stably (green) expressing glioblastoma (U251) and lung cancer (A549) cells in different combinations. Fusion and invasion were monitored using bright field and fluorescence microscopy each day up to 10 days. c′, c′′, f′, f′′, i′ i′′, l′, l′′ At day 10, confocal imaging revealed tumor cell invasion into the FOSs, here shown at two different focal depths of the same fused sphere/spheroids pair. DAPI (blue) indicates nuclei. m Graph showing the percentage of fusion between cancer cell spheres and FOSs. n Graph showing the percentage of invasion of cancer cells into the FOSs. Scale bars: 200 μm (a-l′′)