Fig. 7
From: Cell-in-cell phenomenon: leukocyte engulfment by non-tumorigenic cells and cancer cell lines
Actomyosin distribution during non-professional phagocytosis. Peripheral blood mononuclear cells are stained with CyTRAK orange before co-incubation with BEAS-2B for 4 h. Nuclei are stained using DAPI. A Myosin staining is performed using a secondary antibody coupled with Alexa Fluor 488. B Actin is visualised using phalloidin. The images shown here are representative of > 150 CIC structures analysed. C Merged X-Y-Z scan of myosin-stained cell-in-cell structure to demonstrate the complete engulfment of the leukocyte. Scale bars indicate 10 μm. Arrowheads indicate ring-like structures; arrows indicate interactions with the cytoskeleton of the engulfing cell